Journal: Experimental and Therapeutic Medicine
Article Title: Peroxisome proliferator‑activated receptor γ alleviates human umbilical vein endothelial cell injury in deep vein thrombosis by blocking endoplasmic reticulum stress
doi: 10.3892/etm.2024.12674
Figure Lengend Snippet: PPARγ weakens the activation of endoplasmic reticulum stress in M-HSA-stimulated HUVECs. The protein expression levels of CHOP, GRP78, p-PERK, PERK, IRE1α and p-IRE1α were detected using western blot analysis. *** P<0.001 vs. HSA; # P<0.05, ## P<0.01 and ### P<0.001 vs. M-HSA + oe-NC. PPARγ, proliferator-activated receptor γ; M-HSA, modified glycated human serum albumin; HUVECs, human umbilical vein endothelial cells; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; p, phosphorylated; PERK, protein kinase (PKR)-like ER kinase; IRE1α, p-inositol requiring enzyme 1α; oe, overexpressing; NC, negative control.
Article Snippet: Following blocking with 5% non-fat milk at room temperature for 2 h, the membranes were incubated with primary antibodies against PPARγ (1:1,000; cat. no. ab178860; Abcam), C/EBP homologous protein (CHOP; 1:1,000; cat. no. 2895; Cell Signaling Technology, Inc.), glucose-regulated protein 78 (GRP78; 1:1,000; cat. no. ab21685; Abcam), phosphorylated (p)-protein kinase (PKR)-like ER kinase (p-PERK; 1:200; cat. no. orb504147; Biorbyt), PERK (1:500; cat. no. orb1294328; Biorbyt), p-inositol requiring enzyme 1α (p-IRE1α; 1:1,000; cat. no. ab243665; Abcam), IRE1α (1:1,000; cat. no. ab37073; Abcam) and GAPDH (1:2,500; cat. no. ab9485; Abcam) at 4˚C overnight.
Techniques: Activation Assay, Expressing, Western Blot, Modification, Negative Control
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